Umbrella (Resident Evil)

The Umbrella Corporation’s existence in the same universe as the Avengers would undoubtedly bring them into conflict with each other – and on more than one level.
For starters, they’re a massive organisation whose size – in spite of them operating in different sectors – would definitely result in them clashing commercially with Tony Stark, but there are also two other major ways in which they would come into conflict.
Umbrella’s activities aren’t exactly ethical. They develop biological weapons that include bringing the dead back to life as zombies (evil, superspeed zombie Quicksilver, anyone?), as well as creating terrifying monsters that it would be awesome to see the Avengers face (seriously, imagine the likes of Nemesis, Axemen and Uber-Lickers taking on the likes of Hulk, Black Widow and Hawkeye!).
Moreover, once the Red Queen – Umbrella’s highly-advanced and self-aware computer security system – found out that the Avengers were against them, it/she would launch an all-out assault on the heroes, which would be awesome to see.
